4-Step Reconstitution Protocol
Inspect the Vial
Check that the vial seal is intact, the lyophilized powder is intact at the bottom, and the COA matches the batch number on the label.
Sterilize
Wipe the vial septum with a 70% isopropyl alcohol swab. Allow 30 seconds to dry. Sterilize the injection syringe.
Add BAC Water
Slowly add 1–3 mL of bacteriostatic water (0.9% benzyl alcohol) along the inside wall of the vial. Do not spray directly onto the powder.
Dissolve
Swirl gently — do not shake. Allow 30–60 seconds for full dissolution. The solution should be clear with no visible particles.
Temperature Ranges by Form
| State | Storage Temp | Stability | Notes |
|---|---|---|---|
| Lyophilized (sealed) | -20 °C | 24+ months | Protect from light & moisture |
| Lyophilized (room temp) | +4 to +25 °C | 2–4 weeks | Acceptable in transit only |
| Reconstituted | +2 to +8 °C | 14–28 days | Peptide-dependent — see COA |
| Reconstituted (frozen) | -20 °C | 3–6 months | Avoid repeated freeze-thaw |
Each freeze-thaw cycle degrades peptide integrity. If you need multiple uses, aliquot reconstituted solution into single-use volumes before freezing. Never refreeze a thawed aliquot.
Bacteriostatic Water vs. Sterile Water
Bacteriostatic water contains 0.9% benzyl alcohol as a preservative — this allows the reconstituted solution to be stored at 2–8 °C for 14–28 days without microbial growth. This is the standard choice for research applications requiring multi-dose vials.
Sterile water contains no preservative. Once a vial is opened or pierced, the solution must be used within 24 hours. Use only when single-use is intended.
Light & Moisture Protection
Most lyophilized peptides are hygroscopic — they absorb water from the air, which accelerates degradation. Always store vials sealed and at the correct temperature.
Calculating Concentration
Concentration (mg/mL) = Peptide mass (mg) ÷ Reconstitution volume (mL)
Example: A 5 mg vial reconstituted with 2 mL of BAC water → 2.5 mg/mL concentration.